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Development of an in-house bovine bone graft for safe clinical use

Virtual - MS Teams 10 MAR 20212 PM

"Development of an in-house bovine bone graft for safe clinical use"

 

By Dr. Aghila Rani Nair
Post-doctoral Research Associate, Wound Healing and Oral Diagnosis Research Group, Sharjah Institute for Medical Research, University of Sharjah, Sharjah, UAE

Date/Time:  Wednesday, March 10, 2021 at 2:00PM
Venue: MS Teams: Click here to join the meeting

 

Abstract

Bone and dentine regeneration is a growing field of research in dentistry. Currently available commercial bone grafts are far from satisfactory in meeting long term safe clinical use owing to less than ideal processing techniques and the presence of remnant xenogeneic cellular antigens. The present study is aimed at developing a biomimetic support from bovine bones that has minimal immunological complications when implanted in the recipient. The study is conducted in three phases. In phase I, we investigated the efficacy of two different processing techniques, demineralization and decellularization, for developing the bone grafts and its impact on physical, chemical and biomechanical properties of the bone. In Phase-II, the in house developed bone grafts were studied for its biocompatibility using in-vitro culture systems. In phase-III, the bone regeneration potential of the grafts was evaluated in in vivo models of calvarial defects. Standardization of demineralization and decellularization protocols was done by a series of physico-chemical and physico-chemical and enzymatic treatments respectively. Our results show significant removal of fats, proteins, cellular and genetic material by both the protocols as confirmed by histological, SEM/EDS, FTIR and quantification of total DNA/RNA content. The biocompatibility studies revealed efficient cell attachment, proliferation and osteogenic differentiation. The potential of the in house developed bone grafts to mediate repair of critical size defects in rat calvaria is currently ongoing and preliminary results obtained suggest that decellularized bone samples exhibited enhanced expression of osteogenic markers. The biodegradable, osteoinductive and osteoconductive properties of our in house developed bone grafts as evidenced by in vitro and in vivo experiments suggest the potential for safe clinical use.

 

Brief biography

Aghila Rani Nair joined as a Postdoctoral Research Associate in September 2020 at Research Institute of Medical & Health Sciences (RIMHS). She is associated with the "Wound healing and oral diagnosis research group" led by Prof. AB. Rani Samsudin. She obtained her PhD from the prestigious Sree Chitra Tirunal Institute for Medical Sciences & Technology, Govt. of India. She was awarded the Indian Council of Medical Research Post-doctoral fellowship and is associated with the American Board of Regenerative Medicine (MENA region) in the UAE. Her research interests are in regenerative medicine, bone tissue engineering and exploring the cellular and molecular mechanisms of wound healing and repair.

 



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