A Novel Infection Control Approach Adopted at the University Dental Hospital Sharjah
The Suitability of an Injectable Thermosensitive Polymeric Hydrogel System as Stem Cell Seeding Scaffold
Dr. Wael Taha & Dr. Farah Al-Marzooq
Dental Medicine and Sharjah Institute for Medical Research
University of Sharjah
The strategy in wound healing therapy is an old art that is still poorly understood. Although oral and dental wounds in healthy individuals must heal naturally, critical size defects poses challenges in restoring normal cellular function upon healing.
The first part of the presentation will discuss the suitability of an injectable thermosensitive polymeric hydrogel system as stem cell seeding scaffolds for TMJ tissue regeneration using mixtures of biocompatible materials like chitosan and nanocellulose with calcium chloride and ß-glycerophosphate. Bone marrow derived mesenchymal stem cells (MSC) and dental pulp stem cells were cultured, trypsinised at 80-90% confluence, counted, and passaged at a density of 5–7x105cells/100-mm culture plate then seeded to the hydrogel scaffolds. Chondrogenic differentiation, cell viability and apoptosis were analyzed. The mechanical properties of the hydrogels, immunohistochemistry results and flow cytometric analysis will be deliberated in this presentation. The presentation will also discuss the use of chitosan-based thermosensitive hydrogel use for controlled drug delivery to the TMJ.
The second part of the presentation will discuss a novel infection control approach adopted at the University Dental Hospital Sharjah. Chemical disinfectants have many adverse health effects, including ability to enhance the selection of antibiotic-resistant strains. Therefore, eco-friendly alternatives are required to replace chemical disinfectants. A new probiotic solution containing Bacillus subtilis was applied in a selected dental clinic. There was a significant reduction in the growth of various bacterial species from almost all the surfaces after the application of the probiotic solution. Regular use of probiotics, enriches the environment with beneficial bacteria, makes it less welcoming for harmful bacteria, thus creating healthier ecosystem. The same concept can be applied for treating human diseases including oral infections. This is the ﬁrst study testing the application of a probiotic-based sanitizer in dental settings.