Core facilities

ANIMAL FACILITY

Administrator- Mohamed Elhag Musa (e. mmousa2@sharjah.ac.ae)

Providing state-of-the-art equipments needed for husbandry, breeding and experimentation. Also housing various models of laboratory animals.

ION S5 XL –SEMI CONDUCTOR SEQUENCER- THERMO FISHER SCIENTIFIC

Administrator- Dr. Thenmozhi Venkatachalam (e. tvenkatachalam@sharjah.ac.ae)

The Next Generation Sequencing Ion torrent technology will be used in various fields of scientific research Cancer genomics and transcriptomics, Infectious diseases, Liquid Biopsy Research, Inherited Disease Research, Pharmacogenomics research, Targeted epigenetics of disease, Targeted methylation of different genes involved in various diseases.

ACCESS ARRAY- FLUIDIGM
Administrator- Dr. Thenmozhi Venkatachalam (e. tvenkatachalam@sharjah.ac.ae)

Automate amplicon-based library preparation with the Access Array™ system for up to 48 samples per processing run. Access Array generates next-generation sequencing (NGS)-ready libraries compatible with multiple sequencing platforms including Illumina®, Ion Torrent™, and other NGS systems

QUANT STUDIO 3- qRT PCR- APPLIED BIOSYSTEMS

Administrator- Dr. Thenmozhi Venkatachalam (e. tvenkatachalam@sharjah.ac.ae)

Real-time PCR is used for many applications including targeted gene expression analysis, microRNA analysis, SNP genotyping and CNV analysis.  Gene expression analysis is the most commonly-used application for qPCR.


BIOANALYSER 2100- AGILENT TECHNOLOGIES

Administrator- Dr. Thenmozhi Venkatachalam (e. tvenkatachalam@sharjah.ac.ae)

The Agilent 2100 Bioanalyzer system represents the first microfluidic lab-on-a-chip platform for the electrophoretic analysis of DNA, RNA, and proteins and the flow-cytometric analysis of cells.


THERMAL CYCLER- VERITI- APPLIED BIOSYSTEMS

Administrator- Dr. Thenmozhi Venkatachalam (e. tvenkatachalam@sharjah.ac.ae)

Veriti is a Fast thermal cycler which can run up to six precise and independent temperatures to determine the optimal annealing temperature for cycle sequencing or endpoint PCR. VeriFlex Blocks maintain their thermal characteristics between optimizing and isothermal conditions, minimizing the need optimization. 


QUBIT FLOUROMETER- INVITROGEN

Administrator- Dr. Thenmozhi Venkatachalam (e. tvenkatachalam@sharjah.ac.ae)

The Qubit fluorometer is a small instrument used for quantification of dsDNA, oligos, total RNA, microRNA, and protein, template bead quality of prepared NGS library.  


BIOPLEX-200- BIORAD

Administrator-

The Bio-Plex® 200 system is a suspension array system which offers protein and nucleic acid researchers a reliable multiplex assay solution that permits analysis of up to 100 biomolecules in a single sample.


LS55 FLUORESCENCE SPECTROMETER- PERKIN ELMER

Administrator- Noha Elemam (e. noha.ellemam211@gmail.com)

It can measure fluorescence, phosphorescence, chemi-or bio-luminescence to be used in several applications.


ELX808 ABSORBANCE- BIOTEK

Administrator- Abdul Khader Mohammed (e. amohammed@sharjah.ac.ae)

The ELx808™ Absorbance Microplate Reader offers proven performance for many applications, from endpoint to fast kinetic protocols. Its high quality optical design ensures excellent precision, accuracy and linearity. The ELx808 is well suited to temperature sensitive kinetic assays and many other colorimetric and turbidimetric protocols, such as kinetic ELISA, protein quantification, ELISA, endotoxin analysis and microbial growth assays. Gen5 Software controls the ELx808 for easy data capture and powerful analysis.


NMR 500MHz- BRUKER BIOSPIN

Administrator- Jobi Joseph (e. jjoseph@sharjah.ac.ae)        

Structural determination-Molecular identity- of drug molecules by One Dimensional {Proton-1H,Carbon-13C,Fluorine-19F,Nitrogen-15N  etc.} and Two dimensional(NOESY,COSY,HSQC,HMBC etc.) spectral evaluations.


REAL TIME PCR

Administrator- Dr. Dinesh Kumar (e. ddhanasekaran@sharjah.ac.ae)  

The IX73 inverted microscope system sets new standards in advanced live cell imaging with its compact frame, outstanding optical performance and exceptional flexibility. Manual encoded or semi-motorized options enable a variety of component combinations. The IX73 is available as a one-deck system with a low, ergonomic stage or as a two-deck system with additional expansion capabilities. Both provide the ability to perform a multitude of imaging applications, from fast advanced fluorescence imaging and other demanding techniques to routine testing and documentation.


FLOW CYTOMETER- BD FACSAria III

Administrator- Manju Jayakumar (e. mjayakumar@sharjah.ac.ae)

Immunofluorescence, multicolor phenotyping, Cell cycle, DNA/RNA content analysis, Cell trafficking/ tracking/proliferation ,  Apoptosis, Cell viability, Cytokine analysis, Surface and intracellular marker studies, Changes in intracellular pH, Level of ROS, Lipid, Calcium flux, Sorting. 


CONFOCAL MICROSCOPE- NIKON ECLIPSE TI

Administrator- Manju Jayakumar (e. mjayakumar@sharjah.ac.ae)

High resolution fluorescence imaging, Live cell imaging, Colocalization, Translocation, Timelapse images, Z-stacking/optical sectioning, FRET, FRAP, FLIP.


BD ACCURI C6 FLOW CYTOMETER

Administrator- Dr. Cijo Vazhappilly (e. cvazhappilly@sharjah.ac.ae)  

Apoptosis detection, Cell Cycle Analysis, Protein Expression


BIO-TECHNE, PROTEINSIMPLE WES

Administrator- Varsha Menon (e. vmenon@sharjah.ac.ae)  

Automated Protein expression


VARIOSKAN FLASH-THERMO SCIENTIFIC

Administrator- Dr. Cijo Vazhappilly (e. cvazhappilly@sharjah.ac.ae) 

Plate reader for Luminometric detection, Measuring photometric and fluorescence intensity


MULTISKAN GO- THERMO SCIENTIFIC

Administrator- Dr. Cijo Vazhappilly (e. cvazhappilly@sharjah.ac.ae) 

UV/Vis Microplate reader and Spectrophotometer


HORIZONTAL GEL ELECTROPHORESIS SYSTEMS- CLEAVER SCIENTIFIC 

Administrator- Dr. Farah Al Marzooq (e. falmarzooq@sharjah.ac.ae)

Agarose gel electrophoresis for nucleic acid separation


MICROPULSER™ ELECTROPORATOR- BIORAD 

Administrator- Dr. Farah Al Marzooq (e. falmarzooq@sharjah.ac.ae)

Transformation of bacteria, yeast, and other microorganisms.


PCR THERMAL CYCLER- MASTERCYCLER EP GRADIENT

Administrator- Dr. Farah Al Marzooq (e. falmarzooq@sharjah.ac.ae)

Gradient PCR 


FLOW CYTOMETRY- BD PHARMATRADE

Administrator- Jasmin Abdul Salam (e. jsalam@sharjah.ac.ae)

Supports the multiparametric analysis of individual cells and subpopulations in heterogeneous samples


ELISA MINI WORK STATION

Administrator- Hema Unnikannan (e. hunnikannan@sharjah.ac.ae)

To Perform Automated ELISA Analysis. To take plate optical density reading for various assays in between the wavelength of 405nm to 620nm. 


TISSUE BIOBANK 


TISSUE CULTURE RESEARCH FACILITIES  


DRUG DESIGN and DISCOVERY RESEARCH LABS

Administrator- Srinivasulu Vunnam (e. svunnam@sharjah.ac.ae) 

Furnished with all equipments needed for drug synthesis, purification, identification and biological testing.