Complete List of Major Core Facilities
1. Molecular Imaging Platform
a. Confocal imaging system – 3D molecular devices
This instrument enables researchers to study cells that more closely mimic native conditions, generating more biologically relevant results. In addition, high-content analysis generates multi-parametric data, which is crucial to gaining a better understanding of the complex interactions between molecules and signaling pathways in normal and disease states.
b. RT-PCR instrument:
PCR instrument is used for genetic testing, where a sample of DNA is analyzed for the presence of genetic disease mutations. PCR analysis is also essential to Pre-implantation genetic diagnosis, where individual cells of a developing embryo are tested for mutations. Many forms of cancer involve alterations to oncogenes. By using PCR-based tests is also used to study these mutations.
c. Flow Cytometry(FACS):
Flow Cytometry is a laser-based, biophysical technology employed in cancer research for cell counting, cell cycle analysis, cell sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them by an electronic detection apparatus.
d. Fluorescence Microscopy:
Fluorescent microscopy is often used to: imaging structural components of small specimens, such as cells; conducting viability studies on cell populations; imaging the genetic material within a cell (DNA and RNA); viewing specific cells within a larger population.
The western blot (sometimes called the protein immune-blot) is an analytical technique used to detect specific proteins in the given sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein.
3. Light Inverted microscopes
Inverted microscope is useful for observing living cells or organisms at the bottom of a large container (e.g., a tissue culture flask) under more natural conditions than on a glass slide, as is the case with a conventional microscope.
4. Liquid-Chromatography-Mass Spectrophotometer (LC-MS/MS):
LC/MS systems provide accurate-mass analyses for profiling, identifying, characterizing, and quantifying low molecular-weight compounds and biomolecules. These systems deliver exceptional MS analyses that support demanding applications such as proteomics, metabolomics, impurity testing, product degradation studies, forensics, food safety, and environmental analyses.
5. Tissue culture facility equipment:
Laminar flow hood (biological safety cabinet), CO2 incubator (for most cells), Aspiration pump, Centrifuge, Cold storage rooms (-80 and -20 oC), hypoxia station, Gel electrophoresis, Sterilization equipment, Balances, Vortex, Water purification, pH meter, Magnetic stirrers, Micropipettor(s), Cell counter…etc.
6- Automated ELISA reader and Automated Western Blotting and other equipment needed for
molecular biology research.
7. Organic synthesis laboratory:
State of the art equipment for organic synthesis. The labs are equipped with all necessary tools needed for synthesis, purification and identification of small molecules, among others, automated flash chromatography, HPLC, LCMS, automated evaporating system.
8. State of the art animal facility.
9. 500 MHz NMR facility