Molecular Genetics Laboratory |
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INTRODUCTION
Experiments are designed for students to become familiar with micro pipettes and reagent preparation for use in the preparation of agarose gels to identify human DNA and RNA, plasmids after bacterial cell transformation, PCR products and DNA that has been manipulated by restriction enzymes and ligase for use in southern blots. |
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Equipment
- Autoclave
- Balance
- Water Bath
- Centrifuge
- Micropipettes
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Thermal Cycler
Microwave Heater
UV Transilluminator
Electrophoresis Apparatus |
Experiments
- Calibration
- Making Laboratory Solutions
- DNA Extraction From Whole Blood
- DNA Purification + Gel preparation and Electrophoresis
- Preparation and Transformation of Competent Cell
- Plasmid Isolation
- Use of Restriction Enzymes
- Action of Ligase
- RNA Extraction From Whole Blood
- Polymerase Chain Reaction
- Southern Blot
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Tests AND SERVICES
- Isolation of DNA and RNA From Various Tissues
- Production of cDNA Mutagenesis of Mloned Genes
- Specific Gene Detection Using PCR Technique and Cloning
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