Biochemistry Laboratory
INTRODUCTION |
|
This laboratory introduces students to basic experiments and techniques used in the biochemistry laboratory. Initially, emphasis is placed on the buffer concept and preparation. The principle of kinetic analysis of enzymes is covered with experiments to show the effect of pH, temperature, and substrate concentrations on the activities of enzymes. Experiments on carbohydrates, lipids and vitamins are also conducted. |
 |
equipment
- Spectrophotometers
- Centrifuges
- Automatic Pipettes
|
Water Bath
Glassware and Test Tubes
pH Meters |
EXPERIMENTS
- Basic Calculations, Dilution, and Spectrophotometric Concept, Components, and Utilization (2 Sessions)
- Determination of Unknown Concentration Using Spectrophotometer
- Buffer Preparation and Titration of a Weak Acid With Strong Base
- Effect of pH on the Salivary Amylase Activity
- Effect of the Substrate Concentration Upon the Rate, Velocity, of an Enzymatic Reaction
- Determination of the Iodine Number of Fat
- Estimation of Blood Cholesterol
- Determination of Vitamin C in Various Fruit Drinks
- Alkaline and Acid Hydrolysis of Nucleic Acid
|
Test Services
The test services available in this laboratory are research-based services that utilize state-of-the-art machines and equipment. |
|